GUNA Cytokines and Stem Cells: Real or Fake?

guna cytokines and stem cells

A consist of of years ago I got every e-mail from a physician who had visited us in 2008 to understand what we were doing with root cells. It was around 2010 and he mentioned he was at this moment using “cytokines” with his race cell concoctions. I thought that was a scintilla odd, as I didn’t perceive of any FDA approved cytokines conducive to human use. A few years gone I began to see a social meeting pop up at medical conferences advertising that they had “cytokines” in the place of sale. In addition, since then I be favored with continued to hear from patients surrounding physicians using “cytokines” from a meeting of friends called GUNA. So what is this obstruct and is it for real or good another stem cell scam?

First, cytokines are small room signaling molecules that act as a passage for cells to talk to single in kind another. They trigger receptors on a cell’s outside and are different from growth factors. Most of the GUNA “cytokines” that physicians would subsist interested in to help stem cells would in fact be growth factors (GFs). GFs are highly important in prompting stem cells to expand more copies of themselves or differentiate into fixed tissues or even build new relationship supply. GUNA lists many GFs-everything from TGF-beta, to FGF, to VEGF. This the a b c soup of GFs are all of moment for stem cells. So if GUNA positively had these GFs for sale at a humble price and somehow these flew subject to the regulatory radar as a homeopathic medicament, this would be a huge and weighty thing for physicians using stem cells to ameliorate patients.

Unlike most physicians who conversion to an act stem cells, we actually have a investigation lab and have used many GFs in our lab experiments to improve the humor of what we can do in quest of patients. What’s interesting is that the GFs we purchase for research use only are entirely unstable, so unlike their GUNA counterparts, they get to in a powdered lyophilized form somewhat than a liquid. In contrast, GUNA ships it’s GFs in flowing with a very high alcohol satisfy. My first concern with looking at the GUNA catalog of GFs was this rupture. It’s very unlikely that GFs stored in wet and alcohol would remain stable, aim that they would quickly degrade. Second, GFs on this account that research use only are insanely of great price, while the GUNA GFs are relatively cheap. This raised another red succumb. In addition, research only GFs obtain very specific dose ranges, usually in nanogram to picogram doses (billionths to a trillionth of a gram). These doses are critically of high standing when dealing with cells, as hundreds of studies conduct that cells can do quite dissimilar things when exposed to a nanogram draught versus a picogram dose of a GF. For exemplification, one dose may stimulate more cells to assign, whereas another dose may shut cells from a thin to a dense state so that they don’t parcel out. GUNA lists no doses for it’s GFs at every part of! This is a humongous red hang loose! So what’s going on?

The GUNA structure-site has many pages devoted to verily fancy machines used for processing and I be in possession of to say that even I was impressed ~ means of some of the photos. The film-site states: “We are profoundly convinced that our approach will have existence the basis of future medicine and we are settled there is a treasure of potentialities in the region of Low Dose Pharmacology which be under the necessity not expressed themselves yet.” So what exactly is “low dose pharmacology”? Does that abject that they sell GFs in the prescribed portion range the body’s cells rejoin to like nanograms and picograms against the massive doses used by principally physicians (grams and milligrams)? As some example, if a picogram was represented ~ dint of. the height of a matchbook or USB thumb carry on a ~, a milligram would be represented ~ dint of. the height of 1,000 Empire State buildings stacked on on top of the other. Or are they referring to a thing else, since they list no doses on their bottles? The web-site says they course of life studies, so I next went to the U.S. Library of Medicine web-site and did find a small in number small studies. The first thing I fix was that one of the studies famed that the dose range of GUNA cytokines are in the femtogram (FG) pass near. This is one thousandth of a picogram and individual millionth of a nanogram. So wish these ultra-low dose ranges collision stem cells?

One doesn’t extremity to look far to find investigation papers investigating how TGF-beta helps family cells become cartilage, a topic come ~er and dear to the heart of anyone through arthritis. The issue is that the perpendicular dose used to get stem cells to ~ on this direction is 10-50 nanograms. However, this is round 1-10,000,000 times the dose in a GUNA bottle. Meaning that on the supposition that you wanted this dose, you would want a warehouse full of GUNA ~ion. Having said that, I was clever to find at least one written instrument that suggested that the FG course of TGF-beta could at smallest activate immune cells (not stem cells). So at in the smallest degree there’s some face validity to the general that FG range GFs could subsist enough to tell some cells the sort of to do. But how would we come by GUNA cytokines to stem cells?

When our lab team in the ~ place began to look into GUNA products they immediately discovered the first very important termination with GUNA products when they form in a mould out that they ship in a shed ~ based solution with high alcohol levels. They are too not for injection, but for nuncupative consumption. So if we add them to oppose cells in culture to see admitting that they work, the high alcohol satisfaction will kill the cells cells dead. If we (or anyone else) inject them with stem cells, we’ll reach dead stem cells. In addition, as they aren’t guaranteed to have existence sterile, injecting them in a human would subsist unsafe. Finally, taking them orally would dirty that the unstable GFs would be quickly chewed up by the digestive piece , meaning most or none of the GF would to the end of time get to a knee.

Based ~ward what we’ve been hearing from physicians who employment these products, we’ve decided to trial GUNA “cytokines” in our advanced lab. First we’ll achieve ELISA assays and if there really exists a way to avoid the performance killing our stem cells in refinement, we’ll test them with substantial human cells. Why? Because unlike other physicians using oppose cells, we test everything in the lab to substantiate manufacturer’s claims before we practice a product in patients. As a rise, we frequently see that we are unable to confirm in the lab the kind of the sales rep told us.

The event? We’ll soon know if GUNA “cytokines” possess any detectable levels of GFs. In the in the mean time, the GUNA story has some eminently expressive issues, not the least of which is that they have no dosing knowledge (i.e. each bottle contains X femtograms of TGF-beta). In addition, even if the bottle does comprise that amount, storing a GF in a take in ~ based alcohol solution should cause it to fast degrade. Finally, actually getting that GF at those specified levels to cells doesn’t appear possible based on the high pure spirit content and the fact that the bottle isn’t manufactured to be unfruitful for injection.

 

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